Terra Systems TSI DC® Family of Bioaugmentation Cultures for In-Situ Bioremediation
Dehalococcoides mccartyi is primarily responsible for the complete dechlorination of PCE and TCE to ethene under anaerobic conditions. At sites where Dehalococcoides microorganisms are not present or are found at low numbers, the process will often “stall” at cis-1,2-dichloroethene.
Terra Systems TSI-DC® Bioaugmentation Culture, which contains greater than 1 x 1011 Dehalococcoides/L is injected into the aquifer and will promote the complete dechlorination of PCE or TCE.
TSI-DC® Dehalococcoides mccartyi Bioaugmentation Culture® is an enriched natural bacteria culture that contains Dehalococcoides species for bioaugmentation. This culture dechlorinates tetrachloroethene (PCE) and trichloroethene (TCE) to the non-toxic product ethene. It also can biodegrade carbon tetrachloride and chloroform to methylene chloride and innocuous products. It can be used at sites where bacteria capable of complete reductive dechlorination are not present or there is a need to decrease the remediation time frame. It is estimated that Dehalococcoides are not present in 10 to 40 percent of chlorinated solvent contaminated sites.
Terra Systems 50/50 mix of TSI DC and TSI TCA Dehalococcoides mccartyi/Dehalobacter Bioaugmentation Cultures® is added to the groundwater at sites where the native microorganisms of Dehalococcoides and Dehalobacter are not present, not in sufficient quantity or when the client wants to decrease the remediation time frame for the biodegradation of chlorinated solvents such as tetrachloroethene (PCE), trichloroethene (TCE) and trichloroethane (TCA).
Terra Systems TSI DC RR Rodococus ruber Bioaugmentation Culture® is added to the groundwater at sites where the native microorganisms are not present, are not in sufficient quantity, where the native population does not express all of the required functional genes for 1,4-Dioxane reduction, or when the client wants to decrease the remediation time frame for the biodegradation of 1,4-Dioxane. The TSI DC RR culture is aerobic and requires propane as a cometabolic substrate.
When the situation is required, Terra Systems has Methods for Generation of Anaerobic Dilution Water for TSI DC Bioaugmentation Cultures .
| Product | Differentiation | Application | Benefit | Technical Data Sheet | Safety Data Sheet (SDS) |
|---|---|---|---|---|---|
| TSI DC® Dehalococcoides mccartyi (DHC) | >1 x 1011 DHC cells/L | chlorinated solvents (PCE, TCE) | Required when DHC or vinyl chloride reductase gene are not in sufficient numbers | TSI DC Dehalococcoides mccartyi Bioaugmentation Culture Technical Data Sheet | TSI-DC Dehalococcoides mccartyi Augmentation Culture SDS |
| TSI DC®-TCA Dehalobacter (DHB) | Contains >5 x 1010 Dehalococcoides cells/L and >5 x 1010 Dehalobacter | Trichloroethane to chloroethane | Required when Rhodococcus ruber are not present in sufficient numbers | TSI TCA Dehaobacter Bioaugmentation Culture Technical Data Sheet | TSI TCA Dehalobacter Bioaugmentation Culture SDS |
| Mixed TSI DC® (DHC) and TSI TCA® (DHB) | Contains >5 x 1010 Dehalococcoides/i>< cells/L and >5 x 1010 Dehalobacter | biodegradation of mixed chlorinated solvents such as tetrachloroethene (PCE), trichloroethene (TCE) and trichloroethane (TCA). | Required when Dehalococcoides mccartyi and/or Dehalobacter are not present in sufficient numbers | Mixed TSI DC (DHC) and TSI TCA (DHB) Bioaugmentation Culture Technical Data Sheet | Mixed TSI DC (DHC) and TSI TCA (DHB) Bioaugmentation Culture SDS |
| TSI DC RR Rhodococcus ruber | TSI DC RR® contains ≥1E12 Rhodococcus ruber cells/L. | 1,4-Dioxane | Required when Rhodococcus ruber are not present in sufficient numbers | TSI DC RR Rodococus ruber Data Sheet | TSI DC RR Rodococus ruber SDS |
| Sodium Ascorbate | Non-toxic, food grade reductant to remove oxygen and chlorine. Neutral pH | Injection of Dehalococcoides mccartyi and Dehalobacter Bioaugmentation Cultures | Quickly and efficiently drive chase/dilution water anaerobic | Sodium Acorbate Data Sheet | Sodium Ascorbate SDS |
| pH Buff-Up | Slurry of 50% calcium carbonate in water combined with a proprietary thickener to optimize the pH at a site. | pH optimization at a site. | Maintain optimal pH conditions for reductive dechlorination | pH Buff-Up Technical Data Sheet | pH Buff-Up SDS |
| Buffers | Sodium Bicarbonate, Sodium Carbonate, Magnesium Oxide, Calcium Carbonate | Add to chase/dilution water for low pH (<6.0) aquifers | Maintain optimal pH for microbes | pH Buffers and Buffer Capacity Test Technical Data Sheet | Sodium Bicarbonate SDS Sodium Carbonate Anhydrous SDS Magnesium Oxide SDS Calcium Carbonate SDS |